Table 4.

List of antibodies used for Western blotting.

Antibodies used for Western blottingRRID (−), not found or no exact matchCatalog or clone No.CompanyMono or polyAnimals for antibody productionDilution used
Anti-tubuinNot registeredaProduced by Dr. Fujii, Shinshu UniversityPolyclonalRabbit1/20,000–1/50,000
Anti-α-tubulin(−)RB9281-P0Thermo Fisher Scientific K.K.PolyclonalRabbit1/5,000
Anti-β-tubulinAB_609915T-5201Sigma-AldrichMonoclonalMouse1/1,000
Anti-βIII-tubulin(−)MMS-435PCovanceMonoclonalMouse1/1,000
Anti-β-actinAB_626632sc-47778Santa Cruz Biotechnology Inc.MonoclonalMouse1/5,000
Anti-spectrin (nonerhtyroid) bAB_11214057MAB1622Chemicon InternationalMonoclonalMouse1/250
Anti-PSD-95(−)610495BD Transduction LaboratoriesMonoclonalMouse1/1,000
Anti-α-internexinAB_91800AB5354Chemicon InternationalPolyclonalRabbit1/50,000
Anti-CaMKIIα(−)6G9Chemicon InternationalMonoclonalMouse1/20,000
Anti-CaMKIIβ(−)3232SAGibco BRLMonoclonalMouse1/20,000
Anti-Homer 1AB_1950505GTX103278GeneTex, Inc.PolyclonalRabbit1/20,000
Anti-shank1AB_2270283N22/21UC Davls/NIH NeuroMab facilityMonoclonalMouse1/200
Anti-GKAP (Pan-SAPAP)ABJ0671947N127/31UC Davls/NIH NeuroMab facilityMonoclonalMouse1/250
Anti-ATP5A1, C-termAB_10618791GTX101741GeneTex, Inc.PolyclonalRabbit1/3,000
Anti-ANTAB_671086sc-9299Santa Cruz Biotechnology Inc.PolyclonalGoat1/1,000–1/3,000
Anti-Mouse IgG-HRPOcAB_772210NA931GE HealthcarePolyclonalSheep1/5,000
Anti-Rabbit IgG (H+L)-HRPOcAB_10682917/AB_437787/AB_437787401315Millipore (purchased from Calbiochem)PolyclonalGoat1/20,000–1/50,000
Anti-Goat IgG-HRPOcAB_11214432/AB_92420AP107PChemicon InternationalPolyclonalRabbit1/20,000
  • a Anti-tubulin antibody was produced in rabbit using pig tubulin as antigen, and affinity-purified (Liu et al, 2013).

  • b Anti-fodrin.

  • c HRPO, horseradish peroxidase.