Table 1.

Comparison of protein categories identified in minimum essential cytoskeleton (MEC) and non-MEC.

Protein categoryNo. of proteins
MECNon-MEC
PSD-951
Other Dlg family members6
Other Scaffold/Adaptor proteins14
Cell adhesion proteins9
NT receptors, other receptors, their associated proteins13
Junctional proteins3
Gephyrin1
Tubulin7
Microtubule-related11
α-internexin1
Neurofilament3
Spectrin, spectrin complex17
Dynein-related1
Kinesin, kinesin-like2
Actin11
Actin regulation20
Myosin, myosin-related5
Septins5
CaMKII4
Channels, transporters523
G-protein related4
Small G proteins, their regulators, downstream327
Kinases, phosphatases, their regulators (except for CaMKII)18
Membrane raft7
Membrane trafficking, secretion, cell fusion218
Heat shock proteins, chaperones211
Transcription factors, nuclear proteins8
Ubiquitination11
Extracellular proteins, secreted proteins5
Presynapitc proteins313
Glia, myelin22
Oxidation, reduction14
Metabolic pathway29
Primarily mitochondrial2064
Protein synthesis340
Others10
58366
  • Mass spectrometry analyses were carried out to identify protein components of the three types of PSDL preparations: enriched-type/PSDL (0.75% OG), lean-type/PSDL (1% OG), and initial one/1% OG-IS-11B. Proteins identified in the three preparations were grouped into minimum essential cytoskeleton (MEC) or non-MEC components (Fig 5C). Proteins are clustered into various categories of interest. The number of protein species in each category is listed. Keratins and trypsin are excluded from the list. NT, neurotransmitter. For the protein species in each category, see Tables S3 and S4.