ReagentStock concentrationWork concentrationVolume per 10 ml
Dextran sulphate50%10%2 ml
Ribonucleoside–vanadyl complex200 mM10 mM0.5 ml
SSC buffer20 fold2 fold1 ml
Sodium phosphate buffer pH 71 M50 mM0.5 ml
Formamide100%50%5 ml
DEPC-treated watern/an/a1 ml
Total = 10 ml