Table 2.

Variability of NA (nucleic acid) isolation method across four canine blood samples preserved in “purple top” tubes with EDTA.

NA isolation methodTotal NA (ug) meanTotal NA Std. Dev.NA quality (260/280) meanNA quality (260/280) Std. Dev.High-MW DNA?
PCE1.281.631.753.10Yes
Magmax Core NA purification0.811.021.570.05Yes
Nanobind CBB Big DNA kit2.921.991.850.03Yes
PAXgene Blood DNA kit4.084.851.730.79No
  • DNA from purple top tubes was extracted using either phenol–chloroform extraction (PCE), or three commercial kits (Magmax, Nanobind, and PAXgene). Bold values represent the best performance in a particular category.