Table 1.

Effect of blood sample preservation agent on DNA yield.

Storage agentNA isolation methodInput volume (uL)Output volume (uL)NA conc (ng/uL)Recovered NA total (ng)NA total per mL bloodNA quality (260/280)HMW DNA yielded?
Proprietary (PAXgene)PCE1,7001,0006.376,3703,7472.20Yes
Proprietary (PAXgene)Magmax Core NA purification200902.031839141.66Yes
Proprietary (PAXgene)Nanobind CBB Big DNA kit20010011.101,1105,5501.87Yes
Proprietary (PAXgene)PAXgene Blood DNA kit1,7001,0006.406,4003,7652.38No
EDTA (purple top)PCE1,7001,0000.383802245.21Yes
EDTA (purple top)Magmax Core NA Purification200902.632371,1841.62Yes
EDTA (purple top)Nanobind CBB Big DNA kit20010035.303,53017,6501.84Yes
EDTA (purple top)PAXgene Blood DNA kit1,7001,00010.8010,8006,3531.98No
  • Blood for one canine (Yella) was drawn directly into two tubes containing either a proprietary preservation agent, or EDTA. Three kits were tested against a phenol-chloroform extraction (PCE) standard method. Input and output volumes for each kit are shown, along with actual recovered total DNA mass. NA stands for nucleic acid. EDTA stands for ethylenediaminetetraacetic acid. The extremely high quality (5.21) observed for PCE is likely due to the presence of residual phenol in some samples, which is known to increase the 260/280 ratio beyond the normal quality range.