Table 4.

Oxford Nanopore GridION sequencing run summaries using R9.4.1 flowcells.

Run	Flowcell #	ONT kit	Total bp	Total reads	Read N50	Mean quality (Phred)
1	1	SQK-LSK109	6,274,113,013	658,356	22,619	11.7
1	2	SQK-LSK109^a	7,769,391,385	934,471	18,562	12.2
1	3	SQK-RAD004	6,301,883,845	1,026,445	11,868	11.9
1	4	SQK-RAD004^a	7,573,765,689	1,216,984	12,320	11.3
2	5	SQK-LSK109	4,282,119,674	392,256	35,584	11.38
2	6	SQK-LSK109	4,889,116,279	538,051	26,881	12.07
2	7	SQK-RAD004	6,913,193,761	1,128,659	18,562	10.58
2	8	SQK-RAD004	8,493,017,228	1,830,809	11,868	10.51

SQK-LSK109 is the ligation based library preparation kit. SQK-RAD004 is the transposon based rapid library preparation kit.
↵a Size selection on extracted DNA, before library preparation using the Circulomics short read eliminator kit.