Table 2.

Effects of siRNA knockdowns of Polλ and other TLS Pols on replicative bypass of a cis-syn TT dimer or a (6-4) TT photoproduct carried on the leading DNA strand template in XPV HFs or in WT or Polλ−/− MEFs.

CellsUV lesionsiRNANumber of Kan+ coloniesNumber of blue colonies among Kan+TLS (%)a
XPV HFscis-syn TT dimerNC3123410.9
Rev3308175.5
Rev7321185.6
Polλ274165.8
Rev3 + Polλ332195.7
Rev7 + Polλ294165.4
XPV HFs(6-4) TT PPNC4056215.3
Polθ294237.8
Rev3348257.2
Polλ355267.3
Rev3 + Polλ304258.2
Polθ + Polλ278103.6
WT MEFscis-syn TT dimerNC37612031.9
Polλ−/− MEFsNC43210424.1
Rev32957023.7
Polη2482710.9
WT MEFs(6-4) TT PPNC42212830.3
Polλ−/− MEFsNC3905714.6
Rev32764215.2
Rev1388102.6
Polθ408112.7
  • a Statistical analyses of these data are shown in Table S2.