Table 1.

The effects of siRNA knockdowns of Polλ and other TLS Pols on replicative bypass of a cis-syn TT dimer or a (6-4) TT photoproduct carried on the leading or lagging DNA strand template in XPA human fibroblasts.

UV lesionsiRNALeading strandLagging strand
Number of Kan+ coloniesNumber of blue colonies among Kan+TLS (%)aNumber of Kan+ coloniesNumber of blue colonies among Kan+TLS (%)a
cis-syn TT dimerNC53420538.446515232.7
Polη4067217.73896215.9
Polθ4128620.93946015.2
Polκ52815829.94128921.6
Rev343612729.14088119.9
Rev751214628.557012421.8
Polλ50814428.33949022.8
Rev14466614.83664512.3
Polκ + Polλ3165617.75438615.8
Rev3 + Polλ56616228.661212420.3
Polθ + Polλ3065919.34056516.0
Polη + Polλ5626611.7587569.5
Rev1 + Polλ408184.4422153.6
(6-4) TT PPNC63021534.156217431.0
Polλ3866516.84827114.7
Rev34267317.168510014.6
Rev75068216.25418415.5
Rev3 + Polλ4016716.76129415.4
Rev14026415.93666818.6
Polθ4688417.95568815.8
Rev1 + Polλ545285.1504244.8
Polθ + Polλ668375.5635304.7
  • a Statistical analyses of these data are shown in Table S1.