Table 1.

The effects of siRNA knockdowns of Polλ and other TLS Pols on replicative bypass of a cis-syn TT dimer or a (6-4) TT photoproduct carried on the leading or lagging DNA strand template in XPA human fibroblasts.

UV lesion	siRNA	Leading strand			Lagging strand
UV lesion	siRNA	Number of Kan⁺ colonies	Number of blue colonies among Kan⁺	TLS (%)^a	Number of Kan⁺ colonies	Number of blue colonies among Kan⁺	TLS (%)^a
cis-syn TT dimer	NC	534	205	38.4	465	152	32.7
	Polη	406	72	17.7	389	62	15.9
	Polθ	412	86	20.9	394	60	15.2
	Polκ	528	158	29.9	412	89	21.6
	Rev3	436	127	29.1	408	81	19.9
	Rev7	512	146	28.5	570	124	21.8
	Polλ	508	144	28.3	394	90	22.8
	Rev1	446	66	14.8	366	45	12.3
	Polκ + Polλ	316	56	17.7	543	86	15.8
	Rev3 + Polλ	566	162	28.6	612	124	20.3
	Polθ + Polλ	306	59	19.3	405	65	16.0
	Polη + Polλ	562	66	11.7	587	56	9.5
	Rev1 + Polλ	408	18	4.4	422	15	3.6
(6-4) TT PP	NC	630	215	34.1	562	174	31.0
	Polλ	386	65	16.8	482	71	14.7
	Rev3	426	73	17.1	685	100	14.6
	Rev7	506	82	16.2	541	84	15.5
	Rev3 + Polλ	401	67	16.7	612	94	15.4
	Rev1	402	64	15.9	366	68	18.6
	Polθ	468	84	17.9	556	88	15.8
	Rev1 + Polλ	545	28	5.1	504	24	4.8
	Polθ + Polλ	668	37	5.5	635	30	4.7

↵a Statistical analyses of these data are shown in Table S1.