Table 5.

UV-induced mutation frequencies in the cII gene in BBMEF cells expressing a (6-4) PP photolyase, cyclobutane pyrimidine dimer (CPD) photolyase, or no photolyase and treated with siRNA for Polλ or other TLS Pols.

PhotolyasesiRNAUVaPhotoreactivationbMutation frequencyc (×10−5)
(6-4) PP photolyaseNC−+16.1 ± 0.7
Polλ−+16.8 ± 0.7
NC++45.9 ± 1.9
Polη++88.7 ± 1.7
Polθ++17.5 ± 1.1
Polλ++30.1 ± 1.0
Rev3++31.5 ± 1.6
Rev1++58.2 ± 2.7
Rev3 + Polλ++31.8 ± 1.1
Rev1 + Polλ++20.1 ± 1.5
CPD photolyaseNC−+17.9 ± 1.2
Polλ−+19.1 ± 1.1
NC++28.9 ± 1.0
Rev1++18.4 ± 1.6
Polθ++18.4 ± 1.2
Polλ++40.9 ± 1.2
Rev3++39.1 ± 2.4
Rev3 + Polλ++39.6 ± 1.2
Rev1 + Polλ++20.4 ± 1.6
Polθ + Polλ++21.8 ± 1.4
pNeo vector (no photolyase)NC−+14.8 ± 1.0
Polλ−+15.0 ± 1.3
NC++55.5 ± 1.9
Polλ++35.0 ± 1.0
Rev3++36.8 ± 2.9

  • ↵a 5 J/m2 of UVC (254 nm) light.

  • ↵b Photoreactivation with UVA (360 nm) light for 3 h.

  • ↵c Data are represented as mean ± SEM. Mean mutation frequencies and standard error of the mean were calculated from four independent experiments.