Table 7.

The effects of siRNA knockdowns of TLS Pols on replicative bypass of a thymine glycol or a 1, N6-ethenodeoxyadenosine carried on the leading strand template in wild type, Polλ−/−, or Polθ−/− MEFs.

DNA lesionMEFssiRNANumber of Kan+ coloniesNumber of blue colonies among Kan+ coloniesTLS (%)a
TgWTNC3177323.0
Polλ−/−NC4054210.4
Polκ3814110.8
Rev34234811.3
Polθ46261.3
Polθ−/−NC3604211.7
Polλ42861.4
εdAWTNC3227021.7
Polλ−/−NC3043210.5
Polι3373811.3
Rev33884210.8
Polθ334113.3
Polθ−/−NC3413610.6
Polλ401133.2
Rev3312123.8
  • a Statistical analyses of these data are shown in Table S5.