Table 6.

Effects of WT (1–575), catalytically inactive D427A, D429A, or N-terminally deleted (245–575) Polλ on TLS opposite a (6-4) TT photoproduct carried on the leading strand in WT HFs.

DNA lesionVector expressingNumber of Kan+ coloniesNumber of blue colonies among Kan+TLS (%)a
(6-4) TT PPVector control3464513.0
WT (1–575) Polλ43811927.2
D427A, D429A Polλ4185312.7
(245–575) Polλ39610125.5
  • a Statistical analyses of these data are shown in Table S4.