Table 1.

Comparison of recombineering efficiencies of exogenous/synthetic intronization (ESI) mutagenesis and counterselection.

Artificial intronization (ESI)Counterselection
MutationsInsertion (PCR)SequencingTotal %Cassette insertion (PCR)Rescue (PCR)SequencingTotal %
GTSE1 (SXIP)32/507/1044.8%5/87/507/78.8%
GTSE1 (T165A)30/5010/1250.0%5/844/5010/1055.0%
CHC (C)22/1006/717.1%6/841/5010/1061.5%
Average48%80%37.3%67%61%100%41.8%