Table 1.

Selection of reaction perturbations predicted to reduce SARS-CoV-19 biomass production in a human cell with equal or less impact on human lung cell-based metabolic maintenance.

Reaction 1Reaction 2Virus optimaHost optimaPerturbation
ATPS4m7577Knockout
ENO6566Knockout
PGM6667Knockout
CYOR6169Knockout
CYORGAPD2530Knockout
CYORPUNP36069Knockout
CYORASPTA6068Knockout
PGKFTCD5859Knockout
ASPCTr76100Enforcement
CBPS76100Enforcement
DHORD976100Enforcement
DHORTS76100Enforcement
OMPDC76100Enforcement
ORPT76100Enforcement
ASNS178100Enforcement
GLNS84100Enforcement
THRD_L84100Enforcement
LEULEULAPc90100Enforcement
LEULEUPEPT1tc90100Enforcement
ASPCTrDHFR73100Enforcement
CBPSDHFR73100Enforcement
DHORD9DHFR73100Enforcement
DHORTSDHFR73100Enforcement
OMPDCDHFR73100Enforcement
ORPTDHFR73100Enforcement
  • Individual or pairs of reaction perturbations are shown, alongside their predicted effects on SARS-CoV-19 and host as percent of optima without any perturbations. For full results, see Tables S2 and S5. Reactions are identified with the short notation used in the RECON2.2 model and their gene and subsystem associations are given in Table S1. Short notations used are: ATPS4m, ATP synthase; ENO, enolase; PGM, phosphoglycerate mutase; CYOR, ubiquinol-6 cytochrome c reductase; GAPD, glyceraldehyde-3-phosphate dehydrogenase; PUNP3, purine-nucleoside phosphorylase (Guanosine); ASPTA, aspartate transaminase; PGK, phosphoglycerate kinase; ASPCTr, carbamoyltransferase; CBPS, carbamoyl-phosphate synthase; DHORD9, dihydroorotic acid dehydrogenase; DHRTS, dihydroorotase; OMPDC, orotidine-5-phosophate decarboxylase; ORPT, orotate phosphoribosyltransferase; ASNS1, asparagine synthase; GLNS, glutamine synthase; THRD, threonine deaminase.