Table 2.

Total parasite proteins identified in LieEVs using in-solution digestion approach for mass spectrometry.

Parasite protein in LieEVs (In solution approach)
Identified proteinsAccession number
60S ribosomal protein L10, putativeLdBPK_040750.1.1
AAA domain (Cdc48 subfamily), putativeLdBPK_301700.1.1
ActinLdBPK_041250.1.1
α tubulinLdBPK_130330.1.1
ATP-dependent RNA helicase, putativeLdBPK_363150.1.1
Dynein heavy chain, putativeLdBPK_231570.1.1
Elongation factor 1-αLdBPK_170170.1.1
Hypothetical protein, conservedLdBPK_341260.1.1
Hypothetical protein, conservedLdBPK_321730.1.1
Hypothetical protein, conservedLdBPK_110310.1.1
Hypothetical protein, conservedLdBPK_252520.1.1
Hypothetical protein, conservedLdBPK_160990.1.1
Hypothetical protein, conservedLdBPK_030300.1.1
Hypothetical protein, conservedLdBPK_061130.1.1
Hypothetical protein, conservedLdBPK_301340.1.1
Hypothetical protein, conservedLdBPK_364490.1.1
Hypothetical protein, conservedLdBPK_060440.1.1
Hypothetical protein, conservedLdBPK_331100.1.1
Hypothetical protein, conservedLdBPK_260570.1.1
Hypothetical protein, conservedLdBPK_341920.1.1
Hypothetical protein, conservedLdBPK_041160.1.1
Hypothetical protein, unknown functionLdBPK_080920.1.1
Hypothetical protein, unknown functionLdBPK_131440.1.1
Hypothetical protein, unknown functionLdBPK_271720.1.1
Isoleucyl-tRNA synthetase, putativeLdBPK_365870.1.1
Phosphatidylinositol 3-kinase–like proteinLdBPK_020100.1.1
Phosphatidylinositol kinase, putativeLdBPK_301840.1.1
Phosphopantothenate–cysteine ligase, putativeLdBPK_251980.1.1
Present in the outer mitochondrial membrane proteome 22-2LdBPK_333090.1.1
Proteasome α 7 subunit, putativeLdBPK_110240.1.1
Protein of unknown function (DUF3250), putativeLdBPK_231490.1.1
Raptor N-terminal CASPase–like domain containing protein, putativeLdBPK_201280.1.1
RNA helicase, putativeLdBPK_090090.1.1
Serine/threonine protein phosphatase catalytic subunit, putativeLdBPK_280730.1.1
Seryl-tRNA synthetaseLdBPK_110100.1.1
Small GTP-binding protein Rab18, putativeLdBPK_331940.1.1
Ubiquitin-protein ligase, putativeLdBPK_366600.1.1
Vasohibin, putativeLdBPK_270470.1.1
  • TritrypDB ID and protein name are shown. Proteins with a minimum protein identification probability of 95% and two minimum peptides are shown. P-values were calculated using the Fisher exact test and spectral counts for three biological replicates (LieEV n = 3), where a minimum P-value was 0.05.