Primers used in this study.

NameSequenceUse
Spo0E_1_FTGGCGAAAGAGGTGGGATTGART-qPCR of spo0E-like gene (PPTDRAFT_04974)—primers set 1
Spo0E_1_RGGGTACGCTCTGTTGTCTACGART-qPCR of spo0E-like gene (PPTDRAFT_04974)—primers set 1
Spo0E_2_FGAGTTGGATCGGCTCCTTAATRT-qPCR of spo0E-like gene (PPTDRAFT_04974)—primers set 2
Spo0E_2_RCTCGTGTTGCTTCTCTTGGART-qPCR of spo0E-like gene (PPTDRAFT_04974)—primers set 2
gyrB_FGCCAGCGATACATTCCACTATRT-qPCR of gyrB gene for normalization
gyrB_RCACGAGAGCCTTCGACATAAART-qPCR of gyrB gene for normalization
Alo13_FATGGAAAATGCAACCACGATTCUsed to amplify the Alo13 locus for cloning (start of AloR13 ORF)
Alo13_RATCAATAAACCTCCTGTTCGGTGUsed to amplify the Alo13 locus for cloning (end of Spo0E promoter)
Alo13_Gib_FCACCGAACAGGAGGTTTATTGATatgtcaaaaggagaagaactttttacagUsed for Gibson cloning of Alo13_F and Alo13_R amplified fragment (lower case letters correspond to beginning of sfGFP)
Alo13_Gib_RGAACGAATCGTGGTTGCATTTTCCATgtttgtcctccttattagttaatcagcUsed for Gibson cloning of Alo13_F and Alo13_R amplified fragment (lower case letters correspond to RBS sequence following hyper-spank promoter)
AloP13_mut_FTGTGCAAAGACGAAGAAGGTTTTTCUsed for site-directed mutagenesis of AloP13 start codon ATG > ACG
AloP13_mut_RCCCCCTTAATAATAGATTTATAAATTTCUsed for site-directed mutagenesis of AloP13 start codon ATG > ACG