Primers used for site-directed mutagenesis.

Site-directed mutagenesis primerSequence (5′–3′)
K50R (forward)CCAACTGGCACTGGGAGGTCCTTAAGTC
K50R (reverse)GACTTAAGGACCTCCCAGTGCCAGTTGG
R263Q (forward)GGTCTCCCTTGGCTCCCAGCAGAACCTTTG
R263Q (reverse)CAAAGGTTCTGCTGGGAGCCAAGGGAGACC
R263K (forward)GGTCTCCCTTGGCTCCAAGCAGAACCTTTGTG
R263K (reverse)CACAAAGGTTCTGCTTGGAGCCAAGGGAGACC
R263E (forward)GGTCTCCCTTGGCTCCGAGCAGAACCTTTGTG
R263E (reverse)CACAAAGGTTCTGCTCGGAGCCAAGGGAGACC
C267S (forward)CCCGGCAGAACCTTAGTGTAAATGAAGACGTG
C267S (reverse)CACGTCTTCATTTACACTAAGGTTCTGCCGGG
C350S (forward)GAGGCCCGGGCCAGTCCCTATTACGGG
C350S (reverse)CCCGTAATAGGGACTGGCCCGGGCCTC