Table 1.

Results of in vitro training with Bw4 on the missing-self response of KIR3DL1⁺ NK cells.

Donor Allotype		Bw4⁻				% Change (IFNγ)	% Change (CD107a)	Bw4⁺				% Change (IFNγ)	% Change (CD107a)
Training Cell Allotype		Bw4⁻		Bw4⁺				Bw4⁻		Bw4⁺
Type of Response to K562		IFNγ⁺ (%)	CD107a⁺ (%)	IFNγ⁺ (%)	CD107a⁺ (%)			IFNγ⁺ (%)	CD107a⁺ (%)	IFNγ⁺ (%)	CD107a⁺ (%)
Training conditions	Low IL2	10.5	22.4	14.5	22.9	38.1**	2.2	6.9	20.7	11.6	19.1	68.1**	−7.7
Training conditions	High IL2	14.8	19.8	19.7	18.7	33.1**	−5.6	11.9	15.9	22.0	14.8	84.8*	−6.9

NK cells were co-cultured with either Bw4⁺BCL or Bw4⁻BCL in medium with either 100 U/ml rhIL-2 or 500 U/ml rhIL-2. After 5 d, K562 cells were added at a 10:1 E:T ratio, in medium with 500 U/ml rhIL-2 and anti-CD107a. 6 h later, the NK cells were stained and analyzed by flow cytometry. Combined data from three experiments, totaling 25 Bw4⁻ donors and 20 Bw4⁺ donors, are shown. The frequency of either CD107a⁺ or IFNγ⁺ NK cells in the viable KIR3DL1⁺NKG2A⁻LILRB1⁻ NK cell gate is shown. Percent change was calculated as: ([Bw4⁺ training − Bw4⁻ training]/Bw4⁻ training). Bold type indicates a significant statistical result as assessed by Sidak’s multiple comparison test from paired two-way ANOVAs. Asterisks denote the degree of significance: **P < 0.01, *P < 0.05.