Table 1.

Results of in vitro training with Bw4 on the missing-self response of KIR3DL1+ NK cells.

Donor AllotypeBw4% Change (IFNγ)% Change (CD107a)Bw4+% Change (IFNγ)% Change (CD107a)
Training Cell AllotypeBw4Bw4+Bw4Bw4+
Type of Response to K562IFNγ+ (%)CD107a+ (%)IFNγ+ (%)CD107a+ (%)IFNγ+ (%)CD107a+ (%)IFNγ+ (%)CD107a+ (%)
Training conditionsLow IL210.522.414.522.938.1**2.26.920.711.619.168.1**−7.7
High IL214.819.819.718.733.1**−5.611.915.922.014.884.8*−6.9
  • NK cells were co-cultured with either Bw4+BCL or Bw4BCL in medium with either 100 U/ml rhIL-2 or 500 U/ml rhIL-2. After 5 d, K562 cells were added at a 10:1 E:T ratio, in medium with 500 U/ml rhIL-2 and anti-CD107a. 6 h later, the NK cells were stained and analyzed by flow cytometry. Combined data from three experiments, totaling 25 Bw4 donors and 20 Bw4+ donors, are shown. The frequency of either CD107a+ or IFNγ+ NK cells in the viable KIR3DL1+NKG2ALILRB1 NK cell gate is shown. Percent change was calculated as: ([Bw4+ training − Bw4 training]/Bw4 training). Bold type indicates a significant statistical result as assessed by Sidak’s multiple comparison test from paired two-way ANOVAs. Asterisks denote the degree of significance: **P < 0.01, *P < 0.05.