Table 1.

smTIRF values.

KAP1 (nM)τoff,1 (s)% A1τoff,2 (s)% A2kon (M−1s−1) × 106n
0a0.25 ± 0.0387 ± 72.26 ± 1.2213 ± 73.64 ± 1.5610
500.29 ± 0.0589 ± 33.64 ± 0.4711 ± 31.03 ± 0.374
1000.28 ± 0.0285 ± 35.79 ± 1.0315 ± 31.42 ± 0.954
2000.27 ± 0.0487 ± 25.47 ± 0.9313 ± 21.43 ± 0.453
4000.23 ± 0.0286 ± 115.03 ± 0.6514 ± 110.77 ± 0.443
  • Fit results from smTIRF measurements of HP1α in the presence of the indicated concentrations of KAP1. N denotes the number of independent experiments, each contributing >100 kinetic traces.

  • a Values taken from reference (76).