Table 2.

Summary of native MS characterization of KaiC and KaiA–KaiC complex formed in the presence of ATP.

Figure number	Ion series	Assignment				Theoretical mass (D)	Experimental mass (D)	Δm (D)^a	Relative quantity (%)
Figure number	Ion series	Protein complex	ATP number	ADP number	Mg²⁺ number	Theoretical mass (D)	Experimental mass (D)	Δm (D)^a	Relative quantity (%)
Fig 1B	Blue	KaiC_AA6	7	5	12	353,462	353,476 ± 18	−14	56^b
Fig 1B	Purple	KaiC_AA6	7	3	12	352,608	352,593 ± 16	15	44^b
Fig 1D	Blue	KaiC_AA6	7	5	12	353,462	353,461 ± 14	1	—
Fig 1D	Red	KaiC_AA6/KaiA₂	6	5	12	418,449	418,445 ± 20	4	67^b
Fig 1D	Orange	KaiC_AA6/KaiA₂	0	11	12	417,969	417,963 ± 20	6	33^b
Fig 1D	Orange	KaiC_AA6/KaiA₂	5	5	12	417,942	417,963 ± 20	−21	33^b
Fig S1B	Blue	KaiC_DD6	11	0	12	353,901	353,902 ± 11	−1	—
Fig S1B	Blue	KaiC_DD6	6	6	12	353,928	353,902 ± 11	26	—
Fig S1D	Blue	KaiC_DD6	6	6	12	353,928	353,924 ± 9	4	74^b
Fig S1D	Blue	KaiC_DD6	11	0	12	353,901	353,924 ± 9	−23	74^b
Fig S1D	Purple	KaiC_DD6	6	5	12	353,501	353,477 ± 9	24	26^b
Fig S1D	Purple	KaiC_DD6	0	12	12	353,448	353,477 ± 9	−29	26^b
Fig S1D	Red	KaiC_DD6/KaiA₂	2	10	12	419,102	419,102 ± 32	0	—
Fig S1D	Red	KaiC_DD6/KaiA₂	7	4	12	419,075	419,102 ± 32	−27	—
Fig S4A	Blue	KaiC_AA/E77Q6	7	5	12	353,396	353,383 ± 13	13	—
Fig S4B	Blue	KaiC_AA/E77Q6	12	0	12	353,796	353,766 ± 29	30	—
Fig S4B	Red	KaiC_AA/E77Q6/KaiA₂	7	4	12	418,533	418,559 ± 59	−26	—

↵a Δm is the mass difference between the experimental mass and the theoretical mass.
↵b Relative quantity of two ion series are shown.