Table 1.

Kinetic parameters for Ero1 complexes and Erv1p.

Enzyme (1 μM)Activation rate constantsHalftime of activation (min−1)kcat (s−1)KM (μM)Hill constant
k1 (min−1)k2 (min−1)
Ero1α–PDI1.04 ± 0.041.12 ± 0.061.56 ± 0.080.64 ± 0.045.0 ± 0.85.2 ± 0.7
Ero1β–PDI1.11 ± 0.03n.m.1.14 ± 0.051.00 ± 0.027.6 ± 1.23.1 ± 0.5
Erv1pn.m.n.m.n.m.0.41 ± 0.0389 ± 181.0 ± 0.1
  • n.m., not measurable.