Table 1.

Overview and background information of all cell lines generated.

CTRL (hESC)GenderAgeNo. of clonesInduction methodDiff.PAX6-GFP
WIBR3FemaleBlastocyst derived (Lengner et al, 2010)1N/AYesYes
Rutgers ID (iPS lines)GenderAgeCategoryLocation (deletion)Ref. genomeDel. startDel. endSize (bp)No. of clonesInduction methodDiff.PAX6-GFP
MH0148130 (22q13_1)Female (patient 1)35Terminal deletion22q13.3NCBI36, HG1849210245496914324811874RNAYesYes
MH0148137Female12Terminal deletion22q13.3NCBI36, HG184947970549522658429534RNANoNo
MH0148157 (22q13_2)Male (patient 2)11Terminal deletion22q13.3NCBI36, HG184936919049463084938944RNAYesNo
Isogenic ESCsGenderAgeCategoryLocation (deletion)CRIPSR Seq.Clones pickedAssaySize (del.)No of clonesInduction methodDiff.PAX6-GFP
WIBR3-1 (Δ22-1)FemaleN/ATerminal deletion22q13.3Guide I: 5′-TAATGTCACTTTTGCTGCAGTGG-3′30PCR∼10 kb1N/AYesYes
WIBR3-2 (Δ22-2)FemaleN/ATerminal deletion22q13.330PCR∼10 kb1N/AYesYes
WIBR2-1 (Δ22-3)MaleN/ATerminal deletion22q13.3Guide II: 5′-CTGCTTGCCTGGGCTCCAGCTGG-3′30PCR∼10 kb1N/ANoNo