RT Journal Article
SR Electronic
T1 Helminth protein enhances wound healing by inhibiting fibrosis and promoting tissue regeneration
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202302249
DO 10.26508/lsa.202302249
VO 7
IS 11
A1 Lothstein, Katherine E
A1 Chen, Fei
A1 Mishra, Pankaj
A1 Smyth, Danielle J
A1 Wu, Wenhui
A1 Lemenze, Alexander
A1 Kumamoto, Yosuke
A1 Maizels, Rick M
A1 Gause, William C
YR 2024
UL https://www.life-science-alliance.org/content/7/11/e202302249.abstract
AB Skin wound healing due to full thickness wounds typically results in fibrosis and scarring, where parenchyma tissue is replaced with connective tissue. A major advance in wound healing research would be to instead promote tissue regeneration. Helminth parasites express excretory/secretory (ES) molecules, which can modulate mammalian host responses. One recently discovered ES protein, TGF-β mimic (TGM), binds the TGF-β receptor, though likely has other activities. Here, we demonstrate that topical administration of TGM under a Tegaderm bandage enhanced wound healing and tissue regeneration in an in vivo wound biopsy model. Increased restoration of normal tissue structure in the wound beds of TGM-treated mice was observed during mid- to late-stage wound healing. Both accelerated re-epithelialization and hair follicle regeneration were observed. Further analysis showed differential expansion of myeloid populations at different wound healing stages, suggesting recruitment and reprogramming of specific macrophage subsets. This study indicates a role for TGM as a potential therapeutic option for enhanced wound healing.All data are available in the main text or the supplementary materials. scRNAseq data were deposited into the Gene Expression Omnibus database under the accession number GSE272815.