RT Journal Article
SR Electronic
T1 Golgi pH homeostasis stabilizes the lysosomal membrane through <em>N</em>-glycosylation of membrane proteins
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202402677
DO 10.26508/lsa.202402677
VO 7
IS 10
A1 Sou, Yu-shin
A1 Yamaguchi, Junji
A1 Masuda, Keisuke
A1 Uchiyama, Yasuo
A1 Maeda, Yusuke
A1 Koike, Masato
YR 2024
UL https://www.life-science-alliance.org/content/7/10/e202402677.abstract
AB Protein glycosylation plays a vital role in various cellular functions, many of which occur within the Golgi apparatus. The Golgi pH regulator (GPHR) is essential for the proper functioning of the Golgi apparatus. The lysosomal membrane contains highly glycosylated membrane proteins in abundance. This study investigated the role of the Golgi luminal pH in N-glycosylation of lysosomal membrane proteins and the effect of this protein modification on membrane stability using Gphr-deficient MEFs. We showed that Gphr deficiency causes an imbalance in the Golgi luminal pH, resulting in abnormal protein N-glycosylation, indicated by a reduction in sialylated glycans and markedly reduced molecular weight of glycoproteins. Further experiments using FRAP and PLA revealed that Gphr deficiency prevented the trafficking dynamics and proximity condition of glycosyltransferases in the Golgi apparatus. In addition, incomplete N-glycosylation of lysosomal membrane proteins affected lysosomal membrane stability, as demonstrated by the increased susceptibility to lysosomal damage. Thus, this study highlights the critical role of Golgi pH regulation in controlling protein glycosylation and the impact of Golgi dysfunction on lysosomal membrane stability.Data supporting the results of this study are available from the corresponding author upon reasonable request.