PT  - JOURNAL ARTICLE
AU  - Liu, Yu
AU  - Min, Yu
AU  - Liu, Yongxin
AU  - Watanabe, Yoshinori
TI  - Phosphorylation of Rec8 cohesin complexes regulates mono-orientation of kinetochores in meiosis I
AID  - 10.26508/lsa.202302556
DP  - 2024 May 01
TA  - Life Science Alliance
PG  - e202302556
VI  - 7
IP  - 5
4099  - https://www.life-science-alliance.org/content/7/5/e202302556.short
4100  - https://www.life-science-alliance.org/content/7/5/e202302556.full
SO  - Life Sci. Alliance2024 May 01; 7
AB  - In meiosis I, unlike in mitosis, sister kinetochores are captured by microtubules emanating from the same spindle pole (mono-orientation) and centromeric cohesion mediated by cohesin is protected in the following anaphase I. The conserved meiosis-specific kinetochore protein meikin (Moa1 in fission yeast) associates with polo-like kinase: Plo1 and regulates both mono-orientation and cohesion protection. Although the phosphorylation of Rec8-S450 by Plo1 associated with Moa1 plays a key role in cohesion protection, how Moa1-Plo1 regulates mono-orientation remains elusive. Here, we identify Plo1 phosphorylation sites in the cohesin subunits, Rec8 and Psm3. The non-phosphorylatable mutations at these sites showed specific defects in mono-orientation. These results enabled the genetic dissection of meikin functions at the centromeres.