RT Journal Article
SR Electronic
T1 Inflammatory markers for improved recurrent UTI diagnosis in postmenopausal women
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202302323
DO 10.26508/lsa.202302323
VO 7
IS 4
A1 Ebrahimzadeh, Tahmineh
A1 Basu, Ujjaini
A1 Lutz, Kevin C
A1 Gadhvi, Jashkaran
A1 Komarovsky, Jessica V
A1 Li, Qiwei
A1 Zimmern, Philippe E
A1 De Nisco, Nicole J
YR 2024
UL http://www.life-science-alliance.org/content/7/4/e202302323.abstract
AB Recurrent urinary tract infection (rUTI) severely impacts postmenopausal women. The lack of rapid and accurate diagnostic tools is a major obstacle in rUTI management as current gold standard methods have >24-h diagnostic windows. Work in animal models and limited human cohorts have identified robust inflammatory responses activated during UTI. Consequently, urinary inflammatory cytokines secreted during UTI may function as diagnostic biomarkers. This study aimed to identify urinary cytokines that could accurately diagnose UTI in a controlled cohort of postmenopausal women. Women passing study exclusion criteria were classified into no UTI and active rUTI groups, and urinary cytokine levels were measured by immunoassay. Pro-inflammatory cytokines IL-8, IL-18, IL-1β, and monocyte chemoattractant protein-1 were significantly elevated in the active rUTI group, and anti-inflammatory cytokines IL-13 and IL-4 were elevated in women without UTI. We evaluated cytokine diagnostic performance and found that an IL-8, prostaglandin E2, and IL-13 multivariable model had the lowest misclassification rate and highest sensitivity. Our data identify urinary IL-8, prostaglandin E2, and IL-13 as candidate biomarkers that may be useful in the development of immunoassay-based UTI diagnostics.