RT Journal Article
SR Electronic
T1 Mitochondria isolated from lipid droplets of white adipose tissue reveal functional differences based on lipid droplet size
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202301934
DO 10.26508/lsa.202301934
VO 7
IS 2
A1 Brownstein, Alexandra J
A1 Veliova, Michaela
A1 Acin-Perez, Rebeca
A1 Villalobos, Frankie
A1 Petcherski, Anton
A1 Tombolato, Alberto
A1 Liesa, Marc
A1 Shirihai, Orian S
YR 2024
UL http://www.life-science-alliance.org/content/7/2/e202301934.abstract
AB Recent studies in brown adipose tissue (BAT) described a unique subpopulation of mitochondria bound to lipid droplets (LDs), which were termed PeriDroplet Mitochondria (PDM). PDM can be isolated from BAT by differential centrifugation and salt washes. Contrary to BAT, this approach has so far not led to the successful isolation of PDM from white adipose tissue (WAT). Here, we developed a method to isolate PDM from WAT with high yield and purity by an optimized proteolytic treatment that preserves the respiratory function of mitochondria. Using this approach, we show that, contrary to BAT, WAT PDM have lower respiratory and ATP synthesis capacities compared with WAT cytoplasmic mitochondria (CM). Furthermore, by isolating PDM from LDs of different sizes, we found a negative correlation between LD size and the respiratory capacity of their PDM in WAT. Thus, our new isolation method reveals tissue-specific characteristics of PDM and establishes the existence of heterogeneity in PDM function determined by LD size.