RT Journal Article
SR Electronic
T1 Megakaryocytes possess a STING pathway that is transferred to platelets to potentiate activation
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202302211
DO 10.26508/lsa.202302211
VO 7
IS 2
A1 El-Mortada, Firas
A1 Landelouci, Karima
A1 Bertrand-Perron, Samuel
A1 Aubé, Félix-Antoine
A1 Poirier, Amélie
A1 Bidias, Amel
A1 Jourdi, Georges
A1 Welman, Mélanie
A1 Gantier, Michael P
A1 Hamilton, Justin R
A1 Kile, Benjamin
A1 Lordkipanidzé, Marie
A1 Pépin, Geneviève
YR 2024
UL http://www.life-science-alliance.org/content/7/2/e202302211.abstract
AB Platelets display unexpected roles in immune and coagulation responses. Emerging evidence suggests that STING is implicated in hypercoagulation. STING is an adaptor protein downstream of the DNA sensor cyclic GMP-AMP synthase (cGAS) that is activated by cytosolic microbial and self-DNA during infections, and in the context of loss of cellular integrity, to instigate the production of type-I IFN and pro-inflammatory cytokines. To date, whether the cGAS-STING pathway is present in platelets and contributes to platelet functions is not defined. Using a combination of pharmacological and genetic approaches, we demonstrate here that megakaryocytes and platelets possess a functional cGAS-STING pathway. Our results suggest that in megakaryocytes, STING stimulation activates a type-I IFN response, and during thrombopoiesis, cGAS and STING are transferred to proplatelets. Finally, we show that both murine and human platelets contain cGAS and STING proteins, and the cGAS-STING pathway contributes to potentiation of platelet activation and aggregation. Taken together, these observations establish for the first time a novel role of the cGAS-STING DNA sensing axis in the megakaryocyte and platelet lineage.