RT Journal Article
SR Electronic
T1 Cost-effective DNA methylation profiling by FML-seq
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202302326
DO 10.26508/lsa.202302326
VO 6
IS 12
A1 Foley, Joseph W
A1 Zhu, Shirley X
A1 West, Robert B
YR 2023
UL http://www.life-science-alliance.org/content/6/12/e202302326.abstract
AB Current methods for profiling DNA methylation require costly reagents, sequencing, and labor time. We introduce fragmentation at methylated loci and sequencing (FML-seq), a sequencing library protocol that greatly reduces all these costs. Relative to other techniques tested on the same human cell lines, FML-seq produces similar measurements of absolute and differential cytosine methylation at a fraction of the price. FML-seq enables inexpensive, high-throughput experimental designs for large-scale epigenetics research projects.