PT  - JOURNAL ARTICLE
AU  - Foley, Joseph W
AU  - Zhu, Shirley X
AU  - West, Robert B
TI  - Cost-effective DNA methylation profiling by FML-seq
AID  - 10.26508/lsa.202302326
DP  - 2023 Dec 01
TA  - Life Science Alliance
PG  - e202302326
VI  - 6
IP  - 12
4099  - http://www.life-science-alliance.org/content/6/12/e202302326.short
4100  - http://www.life-science-alliance.org/content/6/12/e202302326.full
SO  - Life Sci. Alliance2023 Dec 01; 6
AB  - Current methods for profiling DNA methylation require costly reagents, sequencing, and labor time. We introduce fragmentation at methylated loci and sequencing (FML-seq), a sequencing library protocol that greatly reduces all these costs. Relative to other techniques tested on the same human cell lines, FML-seq produces similar measurements of absolute and differential cytosine methylation at a fraction of the price. FML-seq enables inexpensive, high-throughput experimental designs for large-scale epigenetics research projects.