PT  - JOURNAL ARTICLE
AU  - Hernandez, Juan E
AU  - Llorente, Cristina
AU  - Ma, Shengyun
AU  - Miyamoto, Kiana T
AU  - Sinha, Saptarshi
AU  - Steele, Scarlet
AU  - Xiao, Zihui
AU  - Lai, Ching-Jung
AU  - Zuniga, Elina I
AU  - Ghosh, Pradipta
AU  - Schnabl, Bernd
AU  - Huang, Wendy Jia Men
TI  - The arginine methyltransferase PRMT5 promotes mucosal defense in the intestine
AID  - 10.26508/lsa.202302026
DP  - 2023 Nov 01
TA  - Life Science Alliance
PG  - e202302026
VI  - 6
IP  - 11
4099  - http://www.life-science-alliance.org/content/6/11/e202302026.short
4100  - http://www.life-science-alliance.org/content/6/11/e202302026.full
SO  - Life Sci. Alliance2023 Nov 01; 6
AB  - PRMT5 is a type II arginine methyltransferase abundantly expressed in the colonic epithelium. It is up-regulated in inflammatory bowel disease and colorectal cancer. However, its role in mucosal defense against enteric infection has not been studied. Here, we report that Prmt5 in the murine colon is up-regulated in response to Citrobacter rodentium infection. Pathogen clearance in mice with haploinsufficient expression of Prmt5 is significantly delayed compared with wildtype littermate controls. Transcriptomic analyses further reveal that PRMT5 regulates the expression of canonical crypt goblet cell genes involved in mucus production, assembly, and anti-microbial responses via methyltransferase activity–dependent and –independent mechanisms. Together, these findings uncover PRMT5 as a novel regulator of mucosal defense and a potential therapeutic target for treating intestinal diseases.