PT  - JOURNAL ARTICLE
AU  - Zhao, Bing
AU  - Li, Zhuoxin
AU  - Yu, Shaoqing
AU  - Li, Tingting
AU  - Wang, Wen
AU  - Liu, Ran
AU  - Zhang, Biyu
AU  - Fang, Xiya
AU  - Shen, Yezhuang
AU  - Han, Qiuying
AU  - Xu, Xin
AU  - Wang, Kai
AU  - Gong, Weili
AU  - Li, Tao
AU  - Li, Ailing
AU  - Zhou, Tao
AU  - Li, Weihua
AU  - Li, Teng
TI  - LEF1 enhances β-catenin transactivation through IDR-dependent liquid–liquid phase separation
AID  - 10.26508/lsa.202302118
DP  - 2023 Nov 01
TA  - Life Science Alliance
PG  - e202302118
VI  - 6
IP  - 11
4099  - http://www.life-science-alliance.org/content/6/11/e202302118.short
4100  - http://www.life-science-alliance.org/content/6/11/e202302118.full
SO  - Life Sci. Alliance2023 Nov 01; 6
AB  - Wnt/β-catenin signaling plays a crucial role in cancer development, primarily activated by β-catenin forming a transcription complex with LEF/TCF in the nucleus and initiating the transcription of Wnt target genes. Here, we report that LEF1, a member of the LEF/TCF family, can form intrinsically disordered region (IDR)-dependent condensates with β-catenin both in vivo and in vitro, which is required for β-catenin-dependent transcription. Notably, LEF1 with disrupted IDR lost its promoting activity on tumor proliferation and metastasis, which can be restored by substituting with FUS IDR. Our findings provide new insight into the essential role of liquid–liquid phase separation in Wnt/β-catenin signaling and present a potential new target for cancer therapy.