RT Journal Article
SR Electronic
T1 Dissecting morphogenetic apoptosis through a genetic screen in Drosophila
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202301967
DO 10.26508/lsa.202301967
VO 6
IS 10
A1 Barbaste, Audrey
A1 Schott, Sonia
A1 Benassayag, Corinne
A1 Suzanne, Magali
YR 2023
UL http://www.life-science-alliance.org/content/6/10/e202301967.abstract
AB Apoptosis is an essential cellular process both in normal development and pathological contexts. Screens performed to date have focused on the cell autonomous aspect of the process, deciphering the apoptotic cascade leading to cell destruction through the activation of caspases. However, the nonautonomous aspect of the apoptotic pathway, including signals regulating the apoptotic pattern or those sent by the apoptotic cell to its surroundings, is still poorly understood. Here, we describe an unbiased RNAi-based genetic screen whose goal is to identify elements of the “morphogenetic apoptosis pathway” in an integrated model system, the Drosophila leg. We screened about 1,400 candidates, using adult joint morphology, morphogenetic fold formation, and apoptotic pattern as readouts for the identification of potential apoptosis-related genes. We identified 41 genes potentially involved in specific aspects of morphogenetic apoptosis: (1) regulation of the apoptotic process; (2) formation, extrusion, and elimination of apoptotic bodies; and (3) contribution to morphogenesis downstream of apoptosis.