TY - JOUR T1 - UHRF1 is essential for proper cytoplasmic architecture and function of mouse oocytes and derived embryos JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202301904 VL - 6 IS - 8 SP - e202301904 AU - Shuhei Uemura AU - Shoji Maenohara AU - Kimiko Inoue AU - Narumi Ogonuki AU - Shogo Matoba AU - Atsuo Ogura AU - Mayuko Kurumizaka AU - Kazuo Yamagata AU - Jafar Sharif AU - Haruhiko Koseki AU - Koji Ueda AU - Motoko Unoki AU - Hiroyuki Sasaki Y1 - 2023/08/01 UR - https://www.life-science-alliance.org/content/6/8/e202301904.abstract N2 - Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) is a protein essential for the maintenance of DNA methylation in somatic cells. However, UHRF1 is predominantly localized in the cytoplasm of mouse oocytes and preimplantation embryos, where it may play a role unrelated to the nuclear function. We herein report that oocyte-specific Uhrf1 KO results in impaired chromosome segregation, abnormal cleavage division, and preimplantation lethality of derived embryos. Our nuclear transfer experiment showed that the phenotype is attributable to cytoplasmic rather than nuclear defects of the zygotes. A proteomic analysis of KO oocytes revealed the down-regulation of proteins associated with microtubules including tubulins, which occurred independently of transcriptomic changes. Intriguingly, cytoplasmic lattices were disorganized, and mitochondria, endoplasmic reticulum, and components of the subcortical maternal complex were mislocalized. Thus, maternal UHRF1 regulates the proper cytoplasmic architecture and function of oocytes and preimplantation embryos, likely through a mechanism unrelated to DNA methylation. ER -