PT - JOURNAL ARTICLE AU - Tsuji, Shogo AU - Brace, Cynthia S AU - Yao, Ruiqing AU - Tanie, Yoshitaka AU - Tada, Hirobumi AU - Rensing, Nicholas AU - Mizuno, Seiya AU - Almunia, Julio AU - Kong, Yingyi AU - Nakamura, Kazuhiro AU - Furukawa, Takahisa AU - Ogiso, Noboru AU - Toyokuni, Shinya AU - Takahashi, Satoru AU - Wong, Michael AU - Imai, Shin-ichiro AU - Satoh, Akiko TI - Sleep–wake patterns are altered with age, Prdm13 signaling in the DMH, and diet restriction in mice AID - 10.26508/lsa.202301992 DP - 2023 Jun 01 TA - Life Science Alliance PG - e202301992 VI - 6 IP - 6 4099 - http://www.life-science-alliance.org/content/6/6/e202301992.short 4100 - http://www.life-science-alliance.org/content/6/6/e202301992.full SO - Life Sci. Alliance2023 Jun 01; 6 AB - Old animals display significant alterations in sleep–wake patterns such as increases in sleep fragmentation and sleep propensity. Here, we demonstrated that PR-domain containing protein 13 (Prdm13)+ neurons in the dorsomedial hypothalamus (DMH) are activated during sleep deprivation (SD) in young mice but not in old mice. Chemogenetic inhibition of Prdm13+ neurons in the DMH in young mice promotes increase in sleep attempts during SD, suggesting its involvement in sleep control. Furthermore, DMH-specific Prdm13-knockout (DMH-Prdm13-KO) mice recapitulated age-associated sleep alterations such as sleep fragmentation and increased sleep attempts during SD. These phenotypes were further exacerbated during aging, with increased adiposity and decreased physical activity, resulting in shortened lifespan. Dietary restriction (DR), a well-known anti-aging intervention in diverse organisms, ameliorated age-associated sleep fragmentation and increased sleep attempts during SD, whereas these effects of DR were abrogated in DMH-Prdm13-KO mice. Moreover, overexpression of Prdm13 in the DMH ameliorated increased sleep attempts during SD in old mice. Therefore, maintaining Prdm13 signaling in the DMH might play an important role to control sleep–wake patterns during aging.