RT Journal Article
SR Electronic
T1 Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202301963
DO 10.26508/lsa.202301963
VO 6
IS 6
A1 Crozet, Flora
A1 Letort, Gaëlle
A1 Bulteau, Rose
A1 Da Silva, Christelle
A1 Eichmuller, Adrien
A1 Tortorelli, Anna Francesca
A1 Blévinal, Joséphine
A1 Belle, Morgane
A1 Dumont, Julien
A1 Piolot, Tristan
A1 Dauphin, Aurélien
A1 Coulpier, Fanny
A1 Chédotal, Alain
A1 Maître, Jean-Léon
A1 Verlhac, Marie-Hélène
A1 Clarke, Hugh J
A1 Terret, Marie-Emilie
YR 2023
UL http://www.life-science-alliance.org/content/6/6/e202301963.abstract
AB The oocyte must grow and mature before fertilization, thanks to a close dialogue with the somatic cells that surround it. Part of this communication is through filopodia-like protrusions, called transzonal projections (TZPs), sent by the somatic cells to the oocyte membrane. To investigate the contribution of TZPs to oocyte quality, we impaired their structure by generating a full knockout mouse of the TZP structural component myosin-X (MYO10). Using spinning disk and super-resolution microscopy combined with a machine-learning approach to phenotype oocyte morphology, we show that the lack of Myo10 decreases TZP density during oocyte growth. Reduction in TZPs does not prevent oocyte growth but impairs oocyte-matrix integrity. Importantly, we reveal by transcriptomic analysis that gene expression is altered in TZP-deprived oocytes and that oocyte maturation and subsequent early embryonic development are partially affected, effectively reducing mouse fertility. We propose that TZPs play a role in the structural integrity of the germline–somatic complex, which is essential for regulating gene expression in the oocyte and thus its developmental potential.