PT  - JOURNAL ARTICLE
AU  - Tyler GB Soule
AU  - Carly S Pontifex
AU  - Nicole Rosin
AU  - Matthew M Joel
AU  - Sukyoung Lee
AU  - Minh Dang Nguyen
AU  - Sameer Chhibber
AU  - Gerald Pfeffer
TI  - A protocol for single nucleus RNA-seq from frozen skeletal muscle
AID  - 10.26508/lsa.202201806
DP  - 2023 May 01
TA  - Life Science Alliance
PG  - e202201806
VI  - 6
IP  - 5
4099  - https://www.life-science-alliance.org/content/6/5/e202201806.short
4100  - https://www.life-science-alliance.org/content/6/5/e202201806.full
SO  - Life Sci. Alliance2023 May 01; 6
AB  - Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single-cell RNA sequencing because of their large, multinucleated nature. Here, we report a novel, reliable, and cost-effective method to analyze frozen human skeletal muscle by single-nucleus RNA sequencing. This method yields all expected cell types for human skeletal muscle and works on tissue frozen for long periods of time and with significant pathological changes. Our method is ideal for studying banked samples with the intention of studying human muscle disease.