RT Journal Article
SR Electronic
T1 Novel inhibitors of microtubule organization and phragmoplast formation in diverse plant species
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202201657
DO 10.26508/lsa.202201657
VO 6
IS 5
A1 Kimata, Yusuke
A1 Yamada, Moé
A1 Murata, Takashi
A1 Kuwata, Keiko
A1 Sato, Ayato
A1 Suzuki, Takamasa
A1 Kurihara, Daisuke
A1 Hasebe, Mitsuyasu
A1 Higashiyama, Tetsuya
A1 Ueda, Minako
YR 2023
UL http://www.life-science-alliance.org/content/6/5/e202201657.abstract
AB Cell division is essential for development and involves spindle assembly, chromosome separation, and cytokinesis. In plants, the genetic tools for controlling the events in cell division at the desired time are limited and ineffective owing to high redundancy and lethality. Therefore, we screened cell division–affecting compounds in Arabidopsis thaliana zygotes, whose cell division is traceable without time-lapse observations. We then determined the target events of the identified compounds using live-cell imaging of tobacco BY-2 cells. Subsequently, we isolated two compounds, PD-180970 and PP2, neither of which caused lethal damage. PD-180970 disrupted microtubule (MT) organization and, thus, nuclear separation, and PP2 blocked phragmoplast formation and impaired cytokinesis. Phosphoproteomic analysis showed that these compounds reduced the phosphorylation of diverse proteins, including MT-associated proteins (MAP70) and class II Kinesin-12. Moreover, these compounds were effective in multiple plant species, such as cucumber (Cucumis sativus) and moss (Physcomitrium patens). These properties make PD-180970 and PP2 useful tools for transiently controlling plant cell division at key manipulation nodes conserved across diverse plant species.