TY - JOUR T1 - Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stress JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202201494 VL - 6 IS - 4 SP - e202201494 AU - Mélanie Bodnar-Wachtel AU - Anne-Laure Huber AU - Julie Gorry AU - Sabine Hacot AU - Delphine Burlet AU - Laetitia Gérossier AU - Baptiste Guey AU - Nadège Goutagny AU - Birke Bartosch AU - Elise Ballot AU - Julie Lecuelle AU - Caroline Truntzer AU - François Ghiringhelli AU - Bénédicte F Py AU - Yohann Couté AU - Annabelle Ballesta AU - Sylvie Lantuejoul AU - Janet Hall AU - Agnès Tissier AU - Virginie Petrilli Y1 - 2023/04/01 UR - https://www.life-science-alliance.org/content/6/4/e202201494.abstract N2 - NLRP3 is a pattern recognition receptor with a well-documented role in inducing inflammasome assembly in response to cellular stress. Deregulation of its activity leads to many inflammatory disorders including gouty arthritis, Alzheimer disease, and cancer. Whereas its role in the context of cancer has been mostly explored in the immune compartment, whether NLRP3 exerts functions unrelated to immunity in cancer development remains unexplored. Here, we demonstrate that NLRP3 interacts with the ATM kinase to control the activation of the DNA damage response, independently of its inflammasome activity. NLRP3 down-regulation in both broncho- and mammary human epithelial cells significantly impairs ATM pathway activation, leading to lower p53 activation, and provides cells with the ability to resist apoptosis induced by acute genotoxic stress. Interestingly, NLRP3 expression is down-regulated in non-small cell lung cancers and breast cancers, and its expression positively correlates with patient overall survival. Our findings identify a novel non-immune function for NLRP3 in maintaining genome integrity and strengthen the concept of a functional link between innate immunity and DNA damage sensing pathways to maintain cell integrity. ER -