PT  - JOURNAL ARTICLE
AU  - Tsuchiya, Hiroyoshi
AU  - Fujinoki, Masakatsu
AU  - Azuma, Morio
AU  - Koshimizu, Taka-aki
TI  - Vasopressin V1a receptor and oxytocin receptor regulate murine sperm motility differently
AID  - 10.26508/lsa.202201488
DP  - 2023 Apr 01
TA  - Life Science Alliance
PG  - e202201488
VI  - 6
IP  - 4
4099  - http://www.life-science-alliance.org/content/6/4/e202201488.short
4100  - http://www.life-science-alliance.org/content/6/4/e202201488.full
SO  - Life Sci. Alliance2023 Apr 01; 6
AB  - Specific receptors for the neurohypophyseal hormones, arginine vasopressin (AVP) and oxytocin, are present in the male reproductive organs. However, their exact roles remain unknown. To elucidate the physiological functions of pituitary hormones in male reproduction, this study first focused on the distribution and function of one of the AVP receptors, V1a. In situ hybridization analysis revealed high expression of the Avpr1a in Leydig cells of the testes and narrow/clear cells in the epididymis, with the expression pattern differing from that of the oxytocin receptor (OTR). Notably, persistent motility and highly proportional hyperactivation were observed in spermatozoa from V1a receptor–deficient mice. In contrast, OTR blocking by antagonist atosiban decreased hyperactivation rate. Furthermore, AVP stimulation could alter the extracellular pH mediated by the V1a receptor. The results highlight the crucial role of neurohypophyseal hormones in male reproductive physiology, with potential contradicting roles of V1a and OTR in sperm maturation. Our findings suggest that V1a receptor antagonists are potential therapeutic drugs for male infertility.