TY - JOUR T1 - DSCR1 deficiency ameliorates the Aβ pathology of Alzheimer’s disease by enhancing microglial activity JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202201556 VL - 6 IS - 2 SP - e202201556 AU - Chiyeol Choi AU - Hyerin Kim AU - Jiyoung Oh AU - Chanho Park AU - Min Kim AU - Chu-Sook Kim AU - Jiyoung Park Y1 - 2023/02/01 UR - https://www.life-science-alliance.org/content/6/2/e202201556.abstract N2 - Microglial phagocytosis and clearance are important for the removal of amyloid-β (Aβ) plaques in Alzheimer’s disease (AD). Chronic exposure of microglia to Aβ plaques leads to microglial metabolic dysfunction, and dysregulation of microglia can accelerate the deposition of Aβ plaques and cause learning and memory impairment. Thus, regulating microglial Aβ clearance is crucial for the development of therapeutics for AD-related dementia. Here, Down syndrome critical region 1 (DSCR1) deficiency ameliorated Aβ plaque deposition in the 5xFAD mouse model of AD by altering microglial activity; however, the Aβ synthesis pathway was not affected. DSCR1 deficiency improved spatial learning and memory impairment in 5xFAD mice. Furthermore, DSCR1-deficient microglia exhibited accelerated lysosomal degradation of Aβ after phagocytosis, and BV2 cells with stable knockdown of DSCR1 demonstrated enhanced lysosomal activity. RNA-sequencing analysis showed that the transcriptional signatures associated with responses to IFN-γ were significantly up-regulated in DSCR1-knockdown BV2 cells treated with Aβ. Our data strongly suggest that DSCR1 is a critical mediator of microglial degradation of amyloid plaques and a new potential microglial therapeutic target in AD. ER -