RT Journal Article
SR Electronic
T1 The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regeneration
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202201783
DO 10.26508/lsa.202201783
VO 6
IS 2
A1 Kotaro Hirano
A1 Masaki Tsuchiya
A1 Akifumi Shiomi
A1 Seiji Takabayashi
A1 Miki Suzuki
A1 Yudai Ishikawa
A1 Yuya Kawano
A1 Yutaka Takabayashi
A1 Kaori Nishikawa
A1 Kohjiro Nagao
A1 Eiji Umemoto
A1 Yasuo Kitajima
A1 Yusuke Ono
A1 Keiko Nonomura
A1 Hirofumi Shintaku
A1 Yasuo Mori
A1 Masato Umeda
A1 Yuji Hara
YR 2023
UL https://www.life-science-alliance.org/content/6/2/e202201783.abstract
AB Muscle satellite cells (MuSCs), myogenic stem cells in skeletal muscles, play an essential role in muscle regeneration. After skeletal muscle injury, quiescent MuSCs are activated to enter the cell cycle and proliferate, thereby initiating regeneration; however, the mechanisms that ensure successful MuSC division, including chromosome segregation, remain unclear. Here, we show that PIEZO1, a calcium ion (Ca2+)-permeable cation channel activated by membrane tension, mediates spontaneous Ca2+ influx to control the regenerative function of MuSCs. Our genetic engineering approach in mice revealed that PIEZO1 is functionally expressed in MuSCs and that Piezo1 deletion in these cells delays myofibre regeneration after injury. These results are, at least in part, due to a mitotic defect in MuSCs. Mechanistically, this phenotype is caused by impaired PIEZO1-Rho signalling during myogenesis. Thus, we provide the first concrete evidence that PIEZO1, a bona fide mechanosensitive ion channel, promotes proliferation and regenerative functions of MuSCs through precise control of cell division.