PT - JOURNAL ARTICLE AU - Kotaro Hirano AU - Masaki Tsuchiya AU - Akifumi Shiomi AU - Seiji Takabayashi AU - Miki Suzuki AU - Yudai Ishikawa AU - Yuya Kawano AU - Yutaka Takabayashi AU - Kaori Nishikawa AU - Kohjiro Nagao AU - Eiji Umemoto AU - Yasuo Kitajima AU - Yusuke Ono AU - Keiko Nonomura AU - Hirofumi Shintaku AU - Yasuo Mori AU - Masato Umeda AU - Yuji Hara TI - The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regeneration AID - 10.26508/lsa.202201783 DP - 2023 Feb 01 TA - Life Science Alliance PG - e202201783 VI - 6 IP - 2 4099 - https://www.life-science-alliance.org/content/6/2/e202201783.short 4100 - https://www.life-science-alliance.org/content/6/2/e202201783.full SO - Life Sci. Alliance2023 Feb 01; 6 AB - Muscle satellite cells (MuSCs), myogenic stem cells in skeletal muscles, play an essential role in muscle regeneration. After skeletal muscle injury, quiescent MuSCs are activated to enter the cell cycle and proliferate, thereby initiating regeneration; however, the mechanisms that ensure successful MuSC division, including chromosome segregation, remain unclear. Here, we show that PIEZO1, a calcium ion (Ca2+)-permeable cation channel activated by membrane tension, mediates spontaneous Ca2+ influx to control the regenerative function of MuSCs. Our genetic engineering approach in mice revealed that PIEZO1 is functionally expressed in MuSCs and that Piezo1 deletion in these cells delays myofibre regeneration after injury. These results are, at least in part, due to a mitotic defect in MuSCs. Mechanistically, this phenotype is caused by impaired PIEZO1-Rho signalling during myogenesis. Thus, we provide the first concrete evidence that PIEZO1, a bona fide mechanosensitive ion channel, promotes proliferation and regenerative functions of MuSCs through precise control of cell division.