@article {Hiranoe202201783, author = {Kotaro Hirano and Masaki Tsuchiya and Akifumi Shiomi and Seiji Takabayashi and Miki Suzuki and Yudai Ishikawa and Yuya Kawano and Yutaka Takabayashi and Kaori Nishikawa and Kohjiro Nagao and Eiji Umemoto and Yasuo Kitajima and Yusuke Ono and Keiko Nonomura and Hirofumi Shintaku and Yasuo Mori and Masato Umeda and Yuji Hara}, title = {The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regeneration}, volume = {6}, number = {2}, elocation-id = {e202201783}, year = {2023}, doi = {10.26508/lsa.202201783}, publisher = {Life Science Alliance}, abstract = {Muscle satellite cells (MuSCs), myogenic stem cells in skeletal muscles, play an essential role in muscle regeneration. After skeletal muscle injury, quiescent MuSCs are activated to enter the cell cycle and proliferate, thereby initiating regeneration; however, the mechanisms that ensure successful MuSC division, including chromosome segregation, remain unclear. Here, we show that PIEZO1, a calcium ion (Ca2+)-permeable cation channel activated by membrane tension, mediates spontaneous Ca2+ influx to control the regenerative function of MuSCs. Our genetic engineering approach in mice revealed that PIEZO1 is functionally expressed in MuSCs and that Piezo1 deletion in these cells delays myofibre regeneration after injury. These results are, at least in part, due to a mitotic defect in MuSCs. Mechanistically, this phenotype is caused by impaired PIEZO1-Rho signalling during myogenesis. Thus, we provide the first concrete evidence that PIEZO1, a bona fide mechanosensitive ion channel, promotes proliferation and regenerative functions of MuSCs through precise control of cell division.}, URL = {https://www.life-science-alliance.org/content/6/2/e202201783}, eprint = {https://www.life-science-alliance.org/content/6/2/e202201783.full.pdf}, journal = {Life Science Alliance} }