RT Journal Article
SR Electronic
T1 Loss of cell–cell adhesion triggers cell migration through Rac1-dependent ROS generation
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202201529
DO 10.26508/lsa.202201529
VO 6
IS 2
A1 Chen, Yu-Hsuan
A1 Hsu, Jinn-Yuan
A1 Chu, Ching-Tung
A1 Chang, Yao-Wen
A1 Fan, Jia-Rong
A1 Yang, Muh-Hwa
A1 Chen, Hong-Chen
YR 2023
UL https://www.life-science-alliance.org/content/6/2/e202201529.abstract
AB Epithelial cells usually trigger their “migratory machinery” upon loss of adhesion to their neighbors. This default is important for both physiological (e.g., wound healing) and pathological (e.g., tumor metastasis) processes. However, the underlying mechanism for such a default remains unclear. In this study, we used the human head and neck squamous cell carcinoma (HNSCC) SAS cells as a model and found that loss of cell–cell adhesion induced reactive oxygen species (ROS) generation and vimentin expression, both of which were required for SAS cell migration upon loss of cell–cell adhesion. We demonstrated that Tiam1-mediated Rac1 activation was responsible for the ROS generation through NADPH-dependent oxidases. Moreover, the ROS–Src–STAT3 signaling pathway that led to vimentin expression was important for SAS cell migration. The activation of ROS, Src, and STAT3 was also detected in tumor biopsies from HNSCC patients. Notably, activated STAT3 was more abundant at the tumor invasive front and correlated with metastatic progression of HNSCC. Together, our results unveil a mechanism of how cells trigger their migration upon loss of cell–cell adhesion and highlight an important role of the ROS–Src–STAT3 signaling pathway in the progression of HNSCC.