TY - JOUR T1 - Genome-wide profiling of histone modifications in <em>Plasmodium falciparum</em> using CUT&amp;RUN JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202201778 VL - 6 IS - 1 SP - e202201778 AU - Riward Campelo Morillo AU - Chantal T Harris AU - Kit Kennedy AU - Samuel R Henning AU - Björn FC Kafsack Y1 - 2023/01/01 UR - https://www.life-science-alliance.org/content/6/1/e202201778.abstract N2 - We recently adapted a CUT&amp;RUN protocol for genome-wide profiling of chromatin modifications in the human malaria parasite Plasmodium. Using the step-by-step protocol described below, we were able to generate high-quality profiles of multiple histone modifications using only a small fraction of the cells required for ChIP-seq. Using antibodies against two commonly profiled histone modifications, H3K4me3 and H3K9me3, we show here that CUT&amp;RUN profiling is highly reproducible and closely recapitulates previously published ChIP-seq-based abundance profiles of histone marks. Finally, we show that CUT&amp;RUN requires substantially lower sequencing coverage for accurate profiling compared with ChIP-seq. ER -