TY - JOUR T1 - The conserved Pelado/ZSWIM8 protein regulates actin dynamics by promoting linear actin filament polymerization JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202201484 VL - 5 IS - 12 SP - e202201484 AU - Claudia Molina-Pelayo AU - Patricio Olguin AU - Marek Mlodzik AU - Alvaro Glavic Y1 - 2022/12/01 UR - https://www.life-science-alliance.org/content/5/12/e202201484.abstract N2 - Actin filament polymerization can be branched or linear, which depends on the associated regulatory proteins. Competition for actin monomers occurs between proteins that induce branched or linear actin polymerization. Cell specialization requires the regulation of actin filaments to allow the formation of cell type–specific structures, like cuticular hairs in Drosophila, formed by linear actin filaments. Here, we report the functional analysis of CG34401/pelado, a gene encoding a SWIM domain–containing protein, conserved throughout the animal kingdom, called ZSWIM8 in mammals. Mutant pelado epithelial cells display actin hair elongation defects. This phenotype is reversed by increasing actin monomer levels or by either pushing linear actin polymerization or reducing branched actin polymerization. Similarly, in hemocytes, Pelado is essential to induce filopodia, a linear actin-based structure. We further show that this function of Pelado/ZSWIM8 is conserved in human cells, where Pelado inhibits branched actin polymerization in a cell migration context. In summary, our data indicate that the function of Pelado/ZSWIM8 in regulating actin cytoskeletal dynamics is conserved, favoring linear actin polymerization at the expense of branched filaments. ER -