RT Journal Article
SR Electronic
T1 KIF24 depletion induces clustering of supernumerary centrosomes in PDAC cells
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202201470
DO 10.26508/lsa.202201470
VO 5
IS 11
A1 Mashima, Yu
A1 Nohira, Hayato
A1 Sugihara, Hiroki
A1 Dynlacht, Brian David
A1 Kobayashi, Tetsuo
A1 Itoh, Hiroshi
YR 2022
UL http://www.life-science-alliance.org/content/5/11/e202201470.abstract
AB Clustering of supernumerary centrosomes, which potentially leads to cell survival and chromosomal instability, is frequently observed in cancers. However, the molecular mechanisms that control centrosome clustering remain largely unknown. The centrosomal kinesin KIF24 was previously shown to restrain the assembly of primary cilia in mammalian cells. Here, we revealed that KIF24 depletion suppresses multipolar spindle formation by clustering centrosomes in pancreatic ductal adenocarcinoma (PDAC) cells harboring supernumerary centrosomes. KIF24 depletion also induced hyper-proliferation and improved mitotic progression in PDAC cells. In contrast, disruption of primary cilia failed to affect the proliferation and spindle formation in KIF24-depleted cells. These results suggest a novel role for KIF24 in suppressing centrosome clustering independent of primary ciliation in centrosome-amplified PDAC cells.