TY - JOUR T1 - Nuclear ingression of cytoplasmic bodies accompanies a boost in autophagy JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202101160 VL - 5 IS - 9 SP - e202101160 AU - Manon Garcia AU - Sylvain Kumanski AU - Alberto Elías-Villalobos AU - Chantal Cazevieille AU - Caroline Soulet AU - María Moriel-Carretero Y1 - 2022/09/01 UR - https://www.life-science-alliance.org/content/5/9/e202101160.abstract N2 - Membrane contact sites are functional nodes at which organelles reorganize metabolic pathways and adapt to changing cues. In Saccharomyces cerevisiae, the nuclear envelope subdomain surrounding the nucleolus, very plastic and prone to expansion, can establish contacts with the vacuole and be remodeled in response to various metabolic stresses. While using genotoxins with unrelated purposes, we serendipitously discovered a fully new remodeling event at this nuclear subdomain: the nuclear envelope partitions into its regular contact with the vacuole and a dramatic internalization within the nucleus. This leads to the nuclear engulfment of a globular, cytoplasmic portion. In spite of how we discovered it, the phenomenon is likely DNA damage-independent. We define lipids supporting negative curvature, such as phosphatidic acid and sterols, as bona fide drivers of this event. Mechanistically, we suggest that the engulfment of the cytoplasm triggers a suction phenomenon that enhances the docking of proton pump-containing vesicles with the vacuolar membrane, which we show matches a boost in autophagy. Thus, our findings unveil an unprecedented remodeling of the nucleolus-surrounding membranes with impact on metabolic adaptation. ER -