RT Journal Article
SR Electronic
T1 Nuclear ingression of cytoplasmic bodies accompanies a boost in autophagy
JF Life Science Alliance
JO Life Sci. Alliance
FD Life Science Alliance LLC
SP e202101160
DO 10.26508/lsa.202101160
VO 5
IS 9
A1 Manon Garcia
A1 Sylvain Kumanski
A1 Alberto Elías-Villalobos
A1 Chantal Cazevieille
A1 Caroline Soulet
A1 María Moriel-Carretero
YR 2022
UL https://www.life-science-alliance.org/content/5/9/e202101160.abstract
AB Membrane contact sites are functional nodes at which organelles reorganize metabolic pathways and adapt to changing cues. In Saccharomyces cerevisiae, the nuclear envelope subdomain surrounding the nucleolus, very plastic and prone to expansion, can establish contacts with the vacuole and be remodeled in response to various metabolic stresses. While using genotoxins with unrelated purposes, we serendipitously discovered a fully new remodeling event at this nuclear subdomain: the nuclear envelope partitions into its regular contact with the vacuole and a dramatic internalization within the nucleus. This leads to the nuclear engulfment of a globular, cytoplasmic portion. In spite of how we discovered it, the phenomenon is likely DNA damage-independent. We define lipids supporting negative curvature, such as phosphatidic acid and sterols, as bona fide drivers of this event. Mechanistically, we suggest that the engulfment of the cytoplasm triggers a suction phenomenon that enhances the docking of proton pump-containing vesicles with the vacuolar membrane, which we show matches a boost in autophagy. Thus, our findings unveil an unprecedented remodeling of the nucleolus-surrounding membranes with impact on metabolic adaptation.