TY - JOUR T1 - Fluorescent tracking identifies key migratory dendritic cells in the lymph node after radiotherapy JF - Life Science Alliance JO - Life Sci. Alliance DO - 10.26508/lsa.202101337 VL - 5 IS - 9 SP - e202101337 AU - Tiffany C Blair AU - Shelly Bambina AU - Gwen F Kramer AU - Alexa K Dowdell AU - Alejandro F Alice AU - Jason R Baird AU - Amanda W Lund AU - Brian D Piening AU - Marka R Crittenden AU - Michael J Gough Y1 - 2022/09/01 UR - https://www.life-science-alliance.org/content/5/9/e202101337.abstract N2 - Radiation therapy generates extensive cancer cell death capable of promoting tumor-specific immunity. Within the tumor, conventional dendritic cells (cDCs) are known to carry tumor-associated antigens to the draining lymph node (TdLN) where they initiate T-cell priming. How radiation influences cDC migration is poorly understood. Here, we show that immunological efficacy of radiation therapy is dependent on cDC migration in radioimmunogenic tumors. Using photoconvertible mice, we demonstrate that radiation impairs cDC migration to the TdLN in poorly radioimmunogenic tumors. Comparative transcriptional analysis revealed that cDCs in radioimmunogenic tumors express genes associated with activation of endogenous adjuvant signaling pathways when compared with poorly radioimmunogenic tumors. Moreover, an exogenous adjuvant combined with radiation increased the number of migrating cDCs in these poorly radioimmunogenic tumors. Taken together, our data demonstrate that cDC migration play a critical role in the response to radiation therapy. ER -